5 Simple Statements About Stem cell Explained
5 Simple Statements About Stem cell Explained
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Do you do have a protocol or prompt antibodies to complete ICC staining on my entirely differentiated ALI cultures?
Very best final results are obtained when limiting extended cell exposure to ambient temperature conditions. Take into consideration keeping unused cells in a very humidified incubator with 5% CO2 at 37°C when undertaking larger experiments.
If using Gentle Cell Dissociation Reagent (GCDR), the period of GCDR treatment will have to be optimized. Incubate organoids with GCDR for eight -10 minutes at 37°C and make use of a P1000 pipette to gently pipette up and down, and visually inspect if organoids break up.
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Cross-section histology followed by hematoxylin and eosin (H&E) staining to evaluate the thickness from the small or massive airway epithelium
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Use cell counts to different the sample into two tubes – “unstained” and “with viability Stem cell dye”.
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Take out supernatant and resuspend cells in FACS buffer. Notice: It's important to quench the dissociation reagent by utilizing the same or double the volume of the dissociation reagent.